intraperitoneal injection in humans

Blood 118, 330338, doi: 10.1182/blood-2010-12-327353 (2011). Types of injections: Uses, sites, and what to expect - Medical News Today Cell stem cell 5, 5463, doi: 10.1016/j.stem.2009.05.003 (2009). Whole marrow cells are plated in the culture dish and non-adherent cells were removed during passage. DailyMed - PIPERACILLIN AND TAZOBACTAM injection, powder, lyophilized 2021. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). Muller-Ehmsen, J. MSCs were isolated from C57BL/6 mice bone marrow as previously reported62. 2010 May;27(5):735-8. doi: 10.1007/s11095-009-0031-z. Another example of how intraperitoneal injections are used in studies involving rodents is the use of IP for micro-CT contrast enhanced detection of liver tumors. Stem cells and development 21, 21892203, doi: 10.1089/scd.2011.0674 (2012). Stem cell transplantation: the lung barrier. Importantly, we identified different migration fates of MSCs and traced their ultimate location of three administration ways for the first time. Schrepfer, S. et al. Models should begin with local tissue infection, so that bacterial . Mice were sacrificed and colons were collected for histological analysis. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. Female nude mice (Envigo) were anesthetized by intraperitoneal (IP) injection of ketamine/xylazine and immobilized in a stereotactic frame (Stoelting). Subcutaneous. Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. All experimental procedures performed were approved by Animal Welfare and Ethics Committee of the Fourth Military Medical University (FMMU), which are in accordance with the NIH guidelines. Intraperitoneal - an overview | ScienceDirect Topics Vishnubalaji, R., Al-Nbaheen, M., Kadalmani, B., Aldahmash, A. Intraperitoneal injection. These mice were then randomized into 3 groups receiving different MSCs giving therapies. Saroglitazar suppresses the hepatocellular carcinoma induced by Gao, J., Dennis, J. E., Muzic, R. F., Lundberg, M. & Caplan, A. I. Zhu, H. et al. [6] In 1968, melatonin was delivered to rats intraperitoneally in order to study how brain serotonin would be affected in the midbrain. With the computer-assisted software, we calculated the density of collagen fibers (%) of all groups. 81370585, No. Certainly, cells are not microsphere which is structural rigid, they are well acknowledged about their deformation, however, a plenty of experimental data support that a substantial amount of MSCs are entrapped in the lung when following IV route16. The study showed that the number of small droplets decreased after administration of the egg whites, indicating that they have been changed to large droplets. More recently the IP route has been used for chemotherapy in patients with intra-abdominal malignancies, i.e. Human mesenchymal stem cells license adult CD34+ hemopoietic progenitor cells to differentiate into regulatory dendritic cells through activation of the Notch pathway. Blood and marrow stem cell transplants in autoimmune disease. Caplan, A. I. PDF Recommended Dose Volumes for Common Laboratory Animals - IQ Consortium Background. PDF Proper Method for Intraperitoneal Injections - PerkinElmer After 3 passages, spindle-shaped cell colonies gradually predominate in the cultures. Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. Google Scholar. The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: IP injections are used for a variety of reasons: The peritoneum provides a large surface area for absorption of drug (compared with intramuscular (IM) or subcutaneous); can thus inject a larger fluid volume. Sci Rep 6, 30696 (2016). Intraperitoneal injections as an alternative method for micro-CT The ELISA kit for TNFinduced protein 6 was used (CUSABIO Life science, China). Clinical use of bone marrow, bone marrow concentrate and expanded bone marrow mesenchymal stem cells in cartilage disease. Article . Briefly, colonic segments were homogenized at 50mg/mL in phosphate buffer (50mM, pH 6.0) with 0.5% hexadecyltrimethylammonium bromide. In quantification study, the intensity of engrafted cells in IP and AI groups were significant higher than the IV (P=0.004, P=0.0012; Fig. The AI groups exhibited 9.5% and IV group showed 6.7% FoxP3+ cells, respectively (Fig. PDF An improved mouse model of sepsis based on intraperitoneal injections CAS Your peritoneal space. 4A,B). RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio Rad, Hercules, CA). Another study by coating MSCs with VCAM-1 also exhibited an increased MSCs migration to the inflamed colon and promoted tissue repair capacity25. ADS Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Evid Based Complement Alternat Med. Stem cells (Dayton, Ohio) 30, 15751578, doi: 10.1002/stem.1118 (2012). eCollection 2021. Every 1107 cells were incubated with 10mL DiR solution (PBS based containing 3.5g/mL dye and 0.5% ethanol) for 30min at 37C. PMC Fraguas-Snchez AI, Torres-Surez AI, Cohen M, Delie F, Bastida-Ruiz D, Yart L, Martin-Sabroso C, Fernndez-Carballido A. Pharmaceutics. In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. MSCs organ distribution and engraftment was analyzed and quantified by GFP+ MSCs as well as Near-infrared fluorescence imaging. The authors declare no competing financial interests. The Journal of clinical investigation 117, 258269, doi: 10.1172/jci29159 (2007). Summers, R. W. Novel and future medical management of inflammatory bowel disease. In addition, the effects of different dosing modalities (e.g., oral administration and intraperitoneal injection) on the . Krampera, M. et al. Additionally, the clinical translation is another concern. Abstract Intraperitoneal (IP) injection is frequently reported to be as effective as intravenous (IV) injection. ***P<0.001; **P<0.01. supply of IGF-I to adolescent and adult animals or humans is not known. The overall therapeutic factors, MSCs migration and targeting as well as local immunomodulatory cytokines and FoxP3+ cells infiltration were analyzed. Respir Res 15, 118, doi: 10.1186/s12931-014-0118-x (2014). Monitor chest movements to make sure the animal is doing ok. On the mouse you want to make your IP injection in the lower right or left quadrant of abdomen trying to avoid hitting bladder, liver, or other internal organs. However, whether the lymphocytes actually . Intraperitoneal injections may also allow too-rapid entry of bacteria or LPS into the blood. PDF Guidelines for the Research Use of Adjuvants - National Institutes of Google Scholar. The Ki-67+ cells were greatly increased after MSCs treatment. Histopathological comparison of colitis after 3 days MSCs delivery. Li, Y. P. et al. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. The IP and AI groups showed mean 50.4% and 46.7% Ki-67+ cells/crypt respectively. Disclaimer. MSCs induced more Ki-67 positive cells in the lower part of the crypt than the DSS control. Google Scholar. Cell and tissue research 347, 419427, doi: 10.1007/s00441-011-1306-3 (2012). The complications associated with IP injection are major concern. Secondly, as mentioned above, there are plenty of immune cells in peritoneal cavity28, which are indispensable component of MSCs aggregates. Disease recovery was evaluated by histological server score, gross body weight and survival rate. In addition, fewer female mice achieved surgical anesthesia, suggesting a narrow therapeutic window for intraperitoneal KX in female mice. For intraperitoneal administration, restrain the mouse with one hand and tilt the head backward to expose the abdomen. Best way for mesenchymal stem cells transplantation for colitis. Ng, S. C. et al. Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. Garcia-Bosch, O., Ricart, E. & Panes, J. Intraperitoneal administration allows quick reabsorption of large volumes of substances and is the preferred injection route for nonirritant, isotonic solutions [7,11]. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. (CS), Intraperitoneal, Polymicrobial, and Antibiotics available under aCC-BY 4.0 International license. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. -Lactalbumin is an example of a "retired" self-protein because its expression in normal . Nature medicine 18, 618623, doi: 10.1038/nm.2695 (2012). A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. Generally, MSCs treatments tend to decrease the pro-inflammatory cytokine (TNF-, IL-6, IL-1b and IFN-) and increase the anti-inflammatory cytokine (IL-10). Filipe et al. von Bahr, L. et al. Department of Digestive Diseases, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, 710032, China, Min Wang,Cong Liang,Hao Hu,Lin Zhou,Bing Xu,Xin Wang,Ying Han,Yongzhan Nie,Jie Liang&Kaichun Wu, Department of Gastroenterology, Xian Childrens Hospital, 710006, China, Department of Respiratory and Gastroenterology, Second Peoples Hospital, Xian, 710005, China, Department of Gastroenterology, PLA No.5 Hospital, Yinchuan, 750004, China, You can also search for this author in n=68 mice/group; n=3 for the control and naive (health mice) group. Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury. Article IP injection showed better amelioration of colitis and may be an ideal delivery way for MSCs in IBD therapy. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. Unable to load your collection due to an error, Unable to load your delegates due to an error. The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. (A) Scheme of the treatment, the day of MSCs injection was defined as day 0. The transplanted lymphocytes can produce immunoglobulin (Ig), respond to antigens, and survive for months in this chimeric model (hu-PBL SCID). The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. [1] Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis. Metabolite tracing, radioactive uptake experiments, and CRISPR screens demonstrate that FLVCR1 is the major plasma-membrane choline transporter. Development of Pluoronic nanoparticles of fluorocoxib A for endoscopic Adv Exp Med Biol 371A, 6165 (1995). . Intraperitoneal Injection Is Not a Suitable Administration Route for & Dittel, B. N. Isolation of mouse peritoneal cavity cells. Nature biotechnology, doi: 10.1038/nbt.2816 (2014). Subcutaneous Compared with Intraperitoneal Ketamine-Xylazine for Intraosseous. Proliferation index was defined as percent of Ki-67 positive nuclei within the random visual scope. The incidence and impact of IBD is increasing worldwide. After antigen retrieval, the endogenous peroxidase activity was blocked by 1% H2O2 for 10min. Article 2020 Jun 3;37(6):119. doi: 10.1007/s11095-020-02818-8. By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. This finding was consistent with previous published paper23, in which the intraperitoneal MSCs migrating and engraft at inflamed colon were identified by Tc-99m SPECT imaging in TNBS induced colitis. For this reason, more MSCs aggregates would form for IP route and produce higher level of serum TSG-6 under TNF- activation, which help reducing the colon inflammation. There was a dose-dependent decrease in AGT activity in liver tissues of CD-1 mice to 24% of basal levels after four hourly intraperitoneal injections of O 6-methylguanine (110 mg/kg). Estes, J. et al. Methods of euthanasia likely to elicit distress vocalizations or pheromones that other animals could hear or smell should be performed in another location, if transportation distress can be minimized. Depletion of O6-alkylguanine-DNA alkyltransferase activity in mammalian In contrast, MSCs were either infused with the bowel waste (for AI route) or trapped in lung/liver vessels (for IV route)51, where aggregates forming would be affected by unfavorable conditions, such as contaminated microenviroment, limited growth space and hemodynamically unstable in lung/liver vessels.

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